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方法 > 操作方法 > 免疫技术 > 染色质免疫沉淀

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void:inDataset: http://aims.fao.org/aos/agrovoc/void.ttl#Agrovoc

创建的: 2025-04-28T10:19:59

skos:notation: 5c2fc6a8

染色质免疫沉淀  

定义

  • Information A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

    来源: https://lod.nal.usda.gov/nalt/336371

    创建的: 6/1/23

     (en)

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http://aims.fao.org/aos/agrovoc/c_5c2fc6a8

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RDF/XML TURTLE JSON-LD 创建的 2023/6/1, 最后修改 2025/5/13