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TERME PRÉFÉRENTIEL
DÉFINITION
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A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
(en)
Source: https://lod.nal.usda.gov/nalt/336371
Date de création: 6/1/23
CONCEPT GÉNÉRIQUE
EST UTILISÉ DANS
- epigenetics (en)
- immunoprecipitation tests (en)
TRADUCTIONS
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anglais
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arabe
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espagnol
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français
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italien
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swahili
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turc
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ukrainien
URI
http://aims.fao.org/aos/agrovoc/c_5c2fc6a8
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