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yöntem > teknikler > immünolojik teknik > kromatin immünopresipitasyon

TÉRMINO PREFERIDO

void:inDataset: http://aims.fao.org/aos/agrovoc/void.ttl#Agrovoc

Creado: 2023-07-05T15:48:30

skos:notation: 5c2fc6a8

kromatin immünopresipitasyon  

DEFINICIÓN

  • A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing. (en)

CONCEPTO GENÉRICO

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URI

http://aims.fao.org/aos/agrovoc/c_5c2fc6a8

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