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Methode > Technik > immunologische Methode > chromatin immunoprecipitation (en)

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DEFINICIÓN

  • A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

    Fuente: https://lod.nal.usda.gov/nalt/336371

    Creado: 6/1/23

    (en)

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http://aims.fao.org/aos/agrovoc/c_5c2fc6a8

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RDF/XML TURTLE JSON-LD Creado 1/6/23, última modificación 10/1/24