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метады > метад > immunological techniques (en) > chromatin immunoprecipitation (en)

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DEFINITION

  • A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

    Source: https://lod.nal.usda.gov/nalt/336371

    Created: 6/1/23

    (en)

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http://aims.fao.org/aos/agrovoc/c_5c2fc6a8

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RDF/XML TURTLE JSON-LD Created 6/1/23, last modified 1/10/24